Manual Setup

It is possible to configure Virtool without completing the graphical setup process.

Passing the --no-setup argument when running Virtool will skip the setup process and use default configuration values or values from manual configuration sources.

Run

1. After installing Virtool, issue the following command in the Virtool install directory

   ./run

   This will start the application server.

   Use ./run --help to see an overview of additional command line arguments Virtool can accept or read the configuration documentation.

2. Visit the application in the browser at localhost:9950

   By default, the server listens on port 9950. When you visit this address for the first time, you will have to complete a one-time setup. This will allow you to configure the essential settings required for Virtool to run.

   

3. Click the Setup button to start setting up Virtool

Connect to a Proxy

You can skip this step if you don’t use a proxy. If you connect to the internet through a proxy, you can configure it here.

1. View the proxy configuration dialog

   You can enter the address for a proxy server through which Virtool should access the internet.

   

2. Enter the address of a proxy server

   We are using a Squid proxy server running locally as an example.

   

3. Click the Save button

   Virtool will attempt to connect to the internet using the provided proxy. Errors will be returned if there is an issue connecting or authentication with the proxy server.

   You should see something like this if the connection is successful:

   

Connect to MongoDB

MongoDB must be installed and running before you can use Virtool.

Once MongoDB is ready, you can configure a connection to it using a MongoDB connection string and database name. When setup is complete, a new database will be created using the provided name.

1. Review the MongoDB setup dialog

   The placeholder values can be used by clicking Connect without changing the form. You can also specify a different address, authentication, or authentication database for your connection by changing the connection string.

   

2. Fill in the form with your own MongoDB connection parameters

   Here, we changed the default database name to virtool-2. Virtool will return an error if there is already a database with the provided Database Name.

   

3. Click the Connect button

   You will see something like the following when the connection is successful:

   

Set Data Location

The data location is where Virtool stores application data including uploaded Illumina libraries, imported sample data, and reference indexes. The path should be located on a storage device that offers good speed, capacity, and redundancy.

1. Review the data location setup dialog

   By default the path will be set to data and will be created in the Virtool installation directory. This configuration should only be used for testing purposes. Use a path on a separate RAID volume or network attached storage (NAS) to store data securely.

   Paths beginning with / will be assumed to be absolute paths. All other paths will be interpreted relative to the Virtool installation directory.

   

2. Set a data location of your choosing

   

3. Click the Save button

   When the data path has been successfully configured, you should see something like this:

   

Set Watch Location

The primary method for making Illumina FASTQ files available to Virtool for sample creation is by uploading them through the web interface.

It is also possible to set a path accessible to the server that will be watched for new read files. Any FASTQ files dropped in this watch directory will be pulled into Virtool and made available for sample creation.

1. Review the watch location setup dialog

   By default the data path will be set to watch and will be created in the Virtool installation directory.

   

2. Set a watch location of your choosing

   An error will be returned if the location doesn’t exist or Virtool doesn’t have permission to write to the location.

   

3. Click the Save button

   This will save the watch path to your setup configuration. The filesystem will not be modified until the server restarts.

   

Save and Restart

1. Review the setup summary

   This shows what will happen as a result of your setup parameters.

   

2. Click the Save and Restart button

   The server will write a configuration file and restart. You will be directed to create a first user when the restart completes.

   

Create First User

Once the server has been setup, you have to create an first user. This user will be automatically given an administrative role.

1. This form will appear if no users are present in the configured database:

   

2. Fill out the form with a username and password

   This user will be an administrator.

   

3. Click the Create User button

   The application will load and you will be automatically logged in as the new user.